RNA

Part:BBa_K4829021:Design

Designed by: Mrigank Pawagi, Das Labs, Aditya Kamath Ammembal   Group: iGEM23_IISc-Bengaluru   (2023-10-09)


Ribotree optimised sequence for the CDS of BBa_K4829014


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 81
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 81
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 646
    Illegal XhoI site found at 64
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 81
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 497


Design Notes

These sequences were optimised by software. The one thing to keep in mind is that it can't be used to optimise UTRs, as they are, very often, not multiples of three, and Ribotree attempts codon optimisation. (However, in UTRs, there are no codons!)


Source

This part is not derived from any organism to the best of our knowledge

References