RNA
Part:BBa_K4829021:Design
Designed by: Mrigank Pawagi, Das Labs, Aditya Kamath Ammembal Group: iGEM23_IISc-Bengaluru (2023-10-09)
Ribotree optimised sequence for the CDS of BBa_K4829014
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 81
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 81
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 646
Illegal XhoI site found at 64 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 81
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 81
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 497
Design Notes
These sequences were optimised by software. The one thing to keep in mind is that it can't be used to optimise UTRs, as they are, very often, not multiples of three, and Ribotree attempts codon optimisation. (However, in UTRs, there are no codons!)
Source
This part is not derived from any organism to the best of our knowledge